Live cell imaging of HeLa cells: Live cell imaging of HeLa cells stable expressing Cell Cycle-Chromobody (TagRFP). Images from a time series of 22 hours are shown

Cell Cycle-Chromobody signal during the cell cycle: In G1, the Chromobody signal is homogeneously distributed throughout the nucleus and cytoplasm. During S phase it accumulates in the nucleus and visualizes the formation of replication foci. In G2, the foci disappear and the cell divides (mitosis).

Time lapse video: Live cell imaging of the stable cell line HeLa-Cell Cycle Chromobody TagRFP expressing Cell Cycle-Chromobody.

Various lysates were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

WB result of CCAR2 antibody (66497-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCAR2 transfected HEK-293 cells.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66497-1-Ig (CCAR2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 U2OS cells were intracellularly stained with 0.2 ug Anti-Human CCAR2 (66497-1-Ig, Clone:2D10F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Various lysates were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

WB result of CCAR2 antibody (66497-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCAR2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66497-1-Ig (CCAR2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

1X10^6 U2OS cells were intracellularly stained with 0.2 ug Anti-Human CCAR2 (66497-1-Ig, Clone:2D10F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66497-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated CCAR2 antibody (CL594-66497, Clone: 2D10F8 ) at dilution of 1:100.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human CCAR2 (CL594-66497, Clone:2D10F8) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0242 (DBC1/CCAR2 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC0242 (DBC1/CCAR2 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13861-1-AP (CCPG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 13861-1-AP (CCPG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CCPG1 antibody (13861-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

A431 cells were subjected to SDS PAGE followed by western blot with 22142-1-AP (RCC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IP Result of anti-RCC1 (IP:22142-1-AP, 3ug; Detection:22142-1-AP 1:1000) with HEK-293 cells lysate 1000ug.

Immunohistochemical analysis of paraffin-embedded human testis using 22142-1-AP (RCC1 antibody) at dilution of 1:50 (under 40x lens).

HeLa cells were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.